Cell Research

We previously proposal, epithelial cell adhesion molecule positive (EpCAM (+)) of hepatocellular carcinoma (HCC) cells form a population of initiating the tumorigenic cells and liver cell carcinoma specimens line. Here, EpCAM (+) circulating tumor cells (CTC) in liver cell carcinoma patients prospectively determine the effect and prognosis of significanceand resection, their stem cell characteristics for further investigation. From 123 patients with hepatocellular carcinoma of the blood sample testing and 1 month ago after resection.

This study is to evaluate the relationship between the organization and the patient's prognosis BMP7 expression in hepatocellular carcinoma (HCC). BMP7 mRNA expression in liver cell carcinoma characteristics is the use of real time PCR and 30 double fresh frozen liver cell carcinoma tissues and the corresponding not cancerous tissue. BMP7 protein expression in liver cell carcinoma is confirmed using immunohistochemistry in tissue microarray. Finally, BMP7 expression and routine clinical pathology characteristic and the prognosis of patients with hepatocellular carcinoma (HCC). BMP7 mRNA expression and protein in liver cancer cell is far higher than normal liver cells. Our results show that high expression BMP7 in liver cell carcinoma on tumor size (p < 0.001), tumor cell differentiation (p = 0.041), serum AFP (p = 0.007), and tumor stage (p < 0.001). Kaplan meier survival analysis showed that a high expression levels of BMP7 lead to obvious liver cell carcinoma patients prognosis. Multivariate analysis showed that BMP7 expression level is an independent prognostic parameters on the overall survival rate for patients with hepatocellular carcinoma. These findings provide evidence shows that a high expression levels of BMP7 as a specific biomarker for the prognosis of patients with hepatocellular carcinoma (HCC) bad. Therefore, we speculate that may be a potential target BMP7 antiangiogenic therapy of liver cancer.

Using fluorescence in situ hybridization (FISH) analysis, we examined the replication mode of the centromere region (homologous counterpart) and the aneuploidy level of chromosome 17 in the interphase nuclei of phytohaemagglutinin (PHA)-stimulated peripheral blood lymphocytes from (1) patients with hepatocellular carcinoma (HCC); (2) patients with liver cirrhosis (LC) due to hepatitis C viral infection who are individuals at a higher increased risk for HCC; and (3) healthy control participants. We also compared the allelic-replication asynchrony and aneuploidy frequencies with serum alpha-fetoprotein (AFP) levels. We found a significant increase in centromeric replication asynchrony accompanied by a high frequency of aneuploidy in lymphocytes of HCC patients compared with those of LC patients and healthy control participants. These changes are similar to those previously observed in other types of malignancy (hematological, ovarian, prostate, and breast cancer). The cytogenetic alterations of aneuploidy and strong asynchronous replication displayed in the lymphocytes of HCC patients arose from malignancy, as they were associated neither with an increased risk for cancer nor with an infection. The cytogenetic cancer-associated markers observed in patients' lymphocytes appeared to be superior to serum AFP, the marker currently used for HCC. Thus, the cytogenetic cancer-associated markers may be potentially useful in noninvasive cancer detection

Secretion clusterin (sClu) is a kind of disease of protein, played an important role in protecting cells from tumor necrosis factor (TNF) α induced apoptosis. The purpose of this study was to survey the molecular mechanism of the influence sClu TNF induced apoptosis in breast cancer cells. The wild type of p53 expression MCF 7 cell line was designed out excessive performance sClu (MCF 7 / sClu), and mda - m - 231 cell line and mutant p53 and sClu son transfection is the silence of the small interference RNA (mda - m - 231 / sClu small interference RNA). Clusterin overexpression influence, in the cell apoptosis and sensitivity are two TNF α in vitro. Our results show that TNF treatment increase BCL 2 mRNA and protein content in breast cancer cells, which suggests that BCL 2 directly subject to nuclear factor - kappaB (NF - kappaB) in response to TNF α. The induction of the base class library 2 mediated p65 subelement of NF - kappaB. The silence of the small interference RNA mediated BCL 2 lead to increased significantly TNF induced apoptosis. The silence of the sClu in mda - m - 231 / sClu small interference RNA cell abolished TNF mediated NF - kappaB activation and BCL 2 overexpression, and present mda - m - 231 / sClu small interference RNA cell significantly more sensitive, TNF than parent mediated apoptosis. In addition, sClu expression in the MCF 7 / sClu cell mediated TNF NF - kappaB promotion activities and BCL 2 overexpression, and presents MCF 7 / Clu cell significant resistance to TNF α mediated apoptosis. Inhibition of NF - kappaB activity or p65 and BCL 2 expression reverse these effects. The results show that sClu awarded breast cancer cells apoptosis induced resistance of TNF α by NF - kappaB activation and BCL 2 overexpression.

The skin is often damaged by genetic and environmental factors such as smoking, contact xenobiotics, heat, hormonal changes, and ultraviolet ray. These factors will cause skin disease. White rattan is quadrangularis falls. (CQ) had been used in folk medicine to treat skin disease since ancient times. In considering take medicinal values show this genus, this is decided to investigate the antitumor activity of CQ. Extraction get and their phenolic content by in vitro CQ antitumor activity evaluation by using A431 (skin epidermoid carcinoma, humans) cell line. A431 cells are used different extract of CQ in dose dependent way. Five extract, acetone extract demonstrates important anticancer activity in A431 cell line. N-hexane, chloroform, ethyl acetate and methanol extract also showed a cell toxicity, but for a relatively small extent than acetone extract. GI (50) value of the acetone extract was found to 8 μ g ml (1), and GI (50) value of the purification part of acetone extract, known as the AFCQ (active part acetone CQ) A431 cells, were found to be 4.8 u g ml (1). In addition, the mechanism of antitumor activity, AFCQ exhibited by comparing its effect and the standard anti-cancer drugs adriamycin (doxorubicin) through the evaluation of the state mark apoptosis after treatment A431 cells and AFCQ and adriamycin. Burlington BCL 2 ratio along with the release of cytochrome c from the mitochondrial cytoplasm, this is a hallmark of cell apoptosis, also be evaluated. Cleavage of PARP revealed that AFCQ A431 cells apoptosis occurred, reference doxorubicin.

Antisense oligonucleotides (management) has been used for prostate cancer model for growth regulatory proteins, and at least one oligosaccharides (against BCL 2) has reached the clinical test. We've found that in LNCaP cell, mono - and double function management, the relative inhibitory expression, as compensation, the base class library 2 inhibition caspase 3 (apoptosis promoter) activities, and increase the expression of androgen receptor (AR) and p300 and il - co catalyst. In addition, the prostate specific membrane antigen (PSMA) and (may be its regulators) interferon (IFN) rise. 14 protein distributed between regulators of apoptosis, androgen control, differentiation antigen and autocrine mediated growth has been inspection. We put these findings including vascular endothelial growth factor (VEGF), promote angiogenesis, not through the compensation significant change, so do not need additional supervision for repression BCL 2 treatment is effective (such as caspase 3).

Invertebrate lysozyme (I - lyz or destabilase) exist in shrimp. The protein may have a function as a peptidoglycan break enzyme and as a peptide enzyme. Shrimp usually vibrio infection. , gram negative bacteria, as is known to all, c - lyz (similar to the chicken lysozyme) is active against these bacteria. In order to further understand renaturation of rules, we determine the sequences of genes and protein structure I - lyz modeling. In addition, I - lyz expression and c - lyz response lipopolysaccharide (LPS) was studied. Shrimp I - lyz gene is made up of two introns interrupt to standardize joint connection. Expression of shrimp I - lyz is transient cut injection followed by after LPS induced after 6 hours in hepatopancreas. By contrast, c - lyz is raised hepatopancreas 4 h post injection and trimmed in gill. L. vannamei I - lyz does not contain of lysozyme catalytic residues (glycohydrolase) is not isopeptidase activities; However, as is known to all, antibacterial activities not only rely on the enzyme activity of protein. Research invertebrates lysozyme will increase our understanding of the regulatory process defense mechanism.

The study describes the use of selective high PI protein extraction and enrichment of human serum albumin (HSA) modified gold nanoparticles (HSA-AuNPs), lysozyme (LYZ) prior to analysis by capillary electrophoresis (CE)with UV detection. HSA gold nanoparticles can be extracted from a complex matrix LYZ HSA the caprock not only stable gold nanoparticles in high salt environment, but also showed a strong electrostatic attraction LYZ neutral pH conditions. LYZ and other high isoelectric point (pI) of the efficient separation of the protein has been successfully achieved by the cationic polyelectrolyte, poly (dimethyl diallyl ammonium chloride the) (PDDAC) filling, background electrolyte. Capture and HSA-AuNPs LYZ PDDAC filled CE can be directly used for analysis of the extracted LYZ releasing agent is not added to the extractor. Extraction efficiency depends on the gold nanoparticles of the solution pH and concentration of HSA. In the preferred extraction conditions, the signal-to-noise ratio of the detection limit of 3 LYZ of 8nm or less. HSA-AuNPs extraction and PDDAC of a combination of filling the CE has been the application of the analysis LYZ egg white, human milk, and the human tear. In addition, based on the extraction of the NP may be coupled to matrix-assisted desorption / ionization time-of-flight mass spectrometry and sodium dodecyl sulfate - polyacrylamide gel electrophoresis

At the transcriptional level lysozyme (LYZ), surf clams clams the four corners clams of defense components combined exposure to heavy metals and bacterial reaction metallothionein (MT) and superoxide dismutase (SOD) were assessed. First, the length LYZ cDNA containing 808 nucleotides and coding for 194 deduced amino acid sequence is determined from the clam. The multiple alignment a discovery, MvLYZ high recognition and invertebrates LYZs from other mollusks. Next, clams exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, singly or in combination, the seven days. Part of three lines of defense of the cumulative mortality of clams and mRNA expression analysis. The highest mortality occurred in the first seven days of comprehensive treatment. Three gene expression is upregulated in response, compared with the control of the different response time, and the transcription level of treatment; just occurred in response to the combined exposure, rather than a single exposure. Experimental group, MvLYZ the expression and expression of MvSOD reached its peak, in the first three days of comprehensive treatment, MvMT expression peaked in the first five days of heavy metal treatment. In addition, the interactive effects of heavy metals and vice transcription reaction changing the exposure time. Therefore, the joint exposure under the transcriptional regulation of the three genes from a single exposure is much more complicated.