Cell Research

Invertebrate lysozyme (I - lyz or destabilase) exist in shrimp. The protein may have a function as a peptidoglycan break enzyme and as a peptide enzyme. Shrimp usually vibrio infection. , gram negative bacteria, as is known to all, c - lyz (similar to the chicken lysozyme) is active against these bacteria. In order to further understand renaturation of rules, we determine the sequences of genes and protein structure I - lyz modeling. In addition, I - lyz expression and c - lyz response lipopolysaccharide (LPS) was studied. Shrimp I - lyz gene is made up of two introns interrupt to standardize joint connection. Expression of shrimp I - lyz is transient cut injection followed by after LPS induced after 6 hours in hepatopancreas. By contrast, c - lyz is raised hepatopancreas 4 h post injection and trimmed in gill. L. vannamei I - lyz does not contain of lysozyme catalytic residues (glycohydrolase) is not isopeptidase activities; However, as is known to all, antibacterial activities not only rely on the enzyme activity of protein. Research invertebrates lysozyme will increase our understanding of the regulatory process defense mechanism.

The study describes the use of selective high PI protein extraction and enrichment of human serum albumin (HSA) modified gold nanoparticles (HSA-AuNPs), lysozyme (LYZ) prior to analysis by capillary electrophoresis (CE)with UV detection. HSA gold nanoparticles can be extracted from a complex matrix LYZ HSA the caprock not only stable gold nanoparticles in high salt environment, but also showed a strong electrostatic attraction LYZ neutral pH conditions. LYZ and other high isoelectric point (pI) of the efficient separation of the protein has been successfully achieved by the cationic polyelectrolyte, poly (dimethyl diallyl ammonium chloride the) (PDDAC) filling, background electrolyte. Capture and HSA-AuNPs LYZ PDDAC filled CE can be directly used for analysis of the extracted LYZ releasing agent is not added to the extractor. Extraction efficiency depends on the gold nanoparticles of the solution pH and concentration of HSA. In the preferred extraction conditions, the signal-to-noise ratio of the detection limit of 3 LYZ of 8nm or less. HSA-AuNPs extraction and PDDAC of a combination of filling the CE has been the application of the analysis LYZ egg white, human milk, and the human tear. In addition, based on the extraction of the NP may be coupled to matrix-assisted desorption / ionization time-of-flight mass spectrometry and sodium dodecyl sulfate - polyacrylamide gel electrophoresis

At the transcriptional level lysozyme (LYZ), surf clams clams the four corners clams of defense components combined exposure to heavy metals and bacterial reaction metallothionein (MT) and superoxide dismutase (SOD) were assessed. First, the length LYZ cDNA containing 808 nucleotides and coding for 194 deduced amino acid sequence is determined from the clam. The multiple alignment a discovery, MvLYZ high recognition and invertebrates LYZs from other mollusks. Next, clams exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, singly or in combination, the seven days. Part of three lines of defense of the cumulative mortality of clams and mRNA expression analysis. The highest mortality occurred in the first seven days of comprehensive treatment. Three gene expression is upregulated in response, compared with the control of the different response time, and the transcription level of treatment; just occurred in response to the combined exposure, rather than a single exposure. Experimental group, MvLYZ the expression and expression of MvSOD reached its peak, in the first three days of comprehensive treatment, MvMT expression peaked in the first five days of heavy metal treatment. In addition, the interactive effects of heavy metals and vice transcription reaction changing the exposure time. Therefore, the joint exposure under the transcriptional regulation of the three genes from a single exposure is much more complicated.