Cell Research

We previously proposal, epithelial cell adhesion molecule positive (EpCAM (+)) of hepatocellular carcinoma (HCC) cells form a population of initiating the tumorigenic cells and liver cell carcinoma specimens line. Here, EpCAM (+) circulating tumor cells (CTC) in liver cell carcinoma patients prospectively determine the effect and prognosis of significanceand resection, their stem cell characteristics for further investigation. From 123 patients with hepatocellular carcinoma of the blood sample testing and 1 month ago after resection.

This study is to evaluate the relationship between the organization and the patient's prognosis BMP7 expression in hepatocellular carcinoma (HCC). BMP7 mRNA expression in liver cell carcinoma characteristics is the use of real time PCR and 30 double fresh frozen liver cell carcinoma tissues and the corresponding not cancerous tissue. BMP7 protein expression in liver cell carcinoma is confirmed using immunohistochemistry in tissue microarray. Finally, BMP7 expression and routine clinical pathology characteristic and the prognosis of patients with hepatocellular carcinoma (HCC). BMP7 mRNA expression and protein in liver cancer cell is far higher than normal liver cells. Our results show that high expression BMP7 in liver cell carcinoma on tumor size (p < 0.001), tumor cell differentiation (p = 0.041), serum AFP (p = 0.007), and tumor stage (p < 0.001). Kaplan meier survival analysis showed that a high expression levels of BMP7 lead to obvious liver cell carcinoma patients prognosis. Multivariate analysis showed that BMP7 expression level is an independent prognostic parameters on the overall survival rate for patients with hepatocellular carcinoma. These findings provide evidence shows that a high expression levels of BMP7 as a specific biomarker for the prognosis of patients with hepatocellular carcinoma (HCC) bad. Therefore, we speculate that may be a potential target BMP7 antiangiogenic therapy of liver cancer.

Using fluorescence in situ hybridization (FISH) analysis, we examined the replication mode of the centromere region (homologous counterpart) and the aneuploidy level of chromosome 17 in the interphase nuclei of phytohaemagglutinin (PHA)-stimulated peripheral blood lymphocytes from (1) patients with hepatocellular carcinoma (HCC); (2) patients with liver cirrhosis (LC) due to hepatitis C viral infection who are individuals at a higher increased risk for HCC; and (3) healthy control participants. We also compared the allelic-replication asynchrony and aneuploidy frequencies with serum alpha-fetoprotein (AFP) levels. We found a significant increase in centromeric replication asynchrony accompanied by a high frequency of aneuploidy in lymphocytes of HCC patients compared with those of LC patients and healthy control participants. These changes are similar to those previously observed in other types of malignancy (hematological, ovarian, prostate, and breast cancer). The cytogenetic alterations of aneuploidy and strong asynchronous replication displayed in the lymphocytes of HCC patients arose from malignancy, as they were associated neither with an increased risk for cancer nor with an infection. The cytogenetic cancer-associated markers observed in patients' lymphocytes appeared to be superior to serum AFP, the marker currently used for HCC. Thus, the cytogenetic cancer-associated markers may be potentially useful in noninvasive cancer detection