Cell Research

At the transcriptional level lysozyme (LYZ), surf clams clams the four corners clams of defense components combined exposure to heavy metals and bacterial reaction metallothionein (MT) and superoxide dismutase (SOD) were assessed. First, the length LYZ cDNA containing 808 nucleotides and coding for 194 deduced amino acid sequence is determined from the clam. The multiple alignment a discovery, MvLYZ high recognition and invertebrates LYZs from other mollusks. Next, clams exposed to Vibrio parahaemolyticus and a mixture of cadmium and mercury, singly or in combination, the seven days. Part of three lines of defense of the cumulative mortality of clams and mRNA expression analysis. The highest mortality occurred in the first seven days of comprehensive treatment. Three gene expression is upregulated in response, compared with the control of the different response time, and the transcription level of treatment; just occurred in response to the combined exposure, rather than a single exposure. Experimental group, MvLYZ the expression and expression of MvSOD reached its peak, in the first three days of comprehensive treatment, MvMT expression peaked in the first five days of heavy metal treatment. In addition, the interactive effects of heavy metals and vice transcription reaction changing the exposure time. Therefore, the joint exposure under the transcriptional regulation of the three genes from a single exposure is much more complicated.