Cell Research

The skin is often damaged by genetic and environmental factors such as smoking, contact xenobiotics, heat, hormonal changes, and ultraviolet ray. These factors will cause skin disease. White rattan is quadrangularis falls. (CQ) had been used in folk medicine to treat skin disease since ancient times. In considering take medicinal values show this genus, this is decided to investigate the antitumor activity of CQ. Extraction get and their phenolic content by in vitro CQ antitumor activity evaluation by using A431 (skin epidermoid carcinoma, humans) cell line. A431 cells are used different extract of CQ in dose dependent way. Five extract, acetone extract demonstrates important anticancer activity in A431 cell line. N-hexane, chloroform, ethyl acetate and methanol extract also showed a cell toxicity, but for a relatively small extent than acetone extract. GI (50) value of the acetone extract was found to 8 μ g ml (1), and GI (50) value of the purification part of acetone extract, known as the AFCQ (active part acetone CQ) A431 cells, were found to be 4.8 u g ml (1). In addition, the mechanism of antitumor activity, AFCQ exhibited by comparing its effect and the standard anti-cancer drugs adriamycin (doxorubicin) through the evaluation of the state mark apoptosis after treatment A431 cells and AFCQ and adriamycin. Burlington BCL 2 ratio along with the release of cytochrome c from the mitochondrial cytoplasm, this is a hallmark of cell apoptosis, also be evaluated. Cleavage of PARP revealed that AFCQ A431 cells apoptosis occurred, reference doxorubicin.