Cell Research

Build a sensitive electrochemical aptamer sensor (aptasensor) to thrombin detection is described. In the use of the advantages of the equipment based on micro electrode the possibility of work with small sample capacity and enjoy faster mass transit interest rates and low interfacial capacitance than in macroelectrodes. Therefore, gold disk microelectrode array is an attractive sensor selection aptasensors. The morphology of the gold disk array microelectrode is through scanning electron microscopy. The interaction between, thrombin of clotting aptamer in gold disk microelectrode array is differential pulse voltammetry proved that using methylene blue (MB) as a kind of electrochemical indicator. MB adsorption to the aptamers through their guanine base. Introduces the thrombin, it replaced the MB adsorption to the aptamers and bound to them. This leads to reduce MB peak current is also related to concentration of thrombin in a dynamic range across from 10 (5) to 10 (- 12) M. This method can selectively detection thrombin linear and detection limit for 0.143 points.